EFEKTITVITIAS EKSTRAK TIMUN LAUT (HOLOTHURIDAE SP.) DALAM MENURUNKAN SITOKIN INFLAMASI INTERLEUKIN–6 (IL-6) PADA TIKUS WISTAR YANG DIINDUKSI PERIODONTITIS.

Introduction : Chronic periodontitis is a infectious disease that cause inflammation and periodontal structure destruction, loss of attachment, bone loss. Bacteria are the main etiological factor that cause periodontitis, and Red Complex Bacteria is the well known to cause periodontitis. Porphyromonas gingivalis is one of the red complex bacteria. In periodontitis patient, Interleukin – 6 plays an important role in developmental of the disease. Sea cucumber is soft invertebrate from class Holothruidea. Sea cucumber has anti-cancer, antibacterial, antifungal, anti-inflammatory, anti-hypertention, antioxidant and antiviral properties
Material and method : Sea cucumber (Holothrudidae sp.) extract was made by using ethanol solution and then rotavapor was used to get the extract. In this research, Pohrphyromonas gingivalis was induced into rat’s gingiva and the anterior teeth were tied by silk ligature. After 3 days, the rats’ gingiva and pocket were scaled, measured and sea cucumber was given orally for P1 and P2 once a day for three days. After 3 days, K1 and P1 were sacrificed,, the tissue was stored in -20⁰C and after 7 days, the K2 and P2 were also sacrificed and IL-6 was measured using ELISA reader.
Results : we found that pocket depth were reduced after scaled and sea cucumber extract given to the rat, but no significant result was found between these two groups. But the expression of IL – 6 was increased in day 7 after administration of sea cucumber extract orally.
Discussion: sea cucumber contains saponin, PUFA, these compounds can block expression of protaglandins and pro-inflammatory cytokine that can influence that expression of IL- 6. IL- 6 can act as anti-inflammatory and pro-inflammatory cytokine.
Conclusion: The sea cucumber extract can influence the expression of inflammatory cytokine, such as interleukin – 6 (IL- 6)