Primer Screening and Genetic Diversity Analysis of Jabon putih (Anthocephalus cadamba (Roxb) Miq.) based on Random Amplified Polymorphic DNA (RAPD) Markers

Introduction: Jabon putih population has currently decreased because of intensive exploitation and land use conversion. Ge netic diversity is an essential factor in maintaining the existence of a species. Objective: The objective of this study was to determine the genetic relationship in Jabon putih from the genetic resource area of the second region of Seed/Seedling Forest Office in Bellabori, Parangloe, Gowa, based on RAPD markers. Methods: The analysis was carried out using 145 DNA samples from seven populations. Primer screening using randomly se lected twelve DNA observed three polymorphic primers out of 21 screened RAPD primers. Result: The polymorphic primers were OPP-08, OPY-09, and OPD-20. The mean of heterozygosity was 0.46, and that of the highest was detected in Luwu Population and Wajo Population (0.48, respectively). Conclusion: The individuals tended to randomly group (did not group according to their provenances), and consequently, the genetic diversity among populations is high.