The Cytotoxic Activity of Marine Sponge-Derived Fungus Aspergillus nomius NC06 Against HT29 Colon Cancer Cells

The study of natural products from marine-derived fungi has been interesting tense to researchers as drug discovery sources. Marine fungus from West Sumatera,Indonesia repeatedly showed their potential for cytotoxic and antimicrobialactivities. This study aims to determine the cytotoxic activity against HT29 coloncancer cells of each fraction of ethyl acetate extracts from Aspergillus nomius NC06derived from marine sponge Neopetrosia chaliniformis. A. nomius was cultivatedwith rice as a growth medium and extracted with ethyl acetate solvent and evaporatedin vacuo to obtain ethyl acetate extract. Furthermore, the compounds of ethyl acetateextract were separated with the VLC (Vacuum Liquide Chromatography) method.Five fractions were obtained, which further investigated their cytotoxic activityagainst HT29 colon cancer cells by using an MTT assay. The result showed thatfractions I and III were categorized as potential fractions due to their IC50 value of13.12 ± 0.39 μg/mL and 2.59 ± 0.19 μg/mL, respectively. It was also supported byANOVA to measure the effect of each concentration (0.1; 1; 10; 100 μg/mL) of eachfraction on the viability percentage of HT29 cells with p < 0.005.Keywords: Aspergillus nomius, Cytotoxic activity, HT29 colon cancer cell