Inhibition of Streptococcus Sanguis and Streptococcus Mutans in Gingivitis as a Result of The Mechanism of HNP1-3 and hBD-2

Gingivitis is a form of abnormality in periodontal tissue which can be found in the general society. The patient does not realize the clinical difference in gingiva caused by lack of knowledge about dental health and also there has not been a single case of mortality because of gingivitis Sickness of periodontal tissue is experienced by almost all people across the globe and it reaches 50% from total adult population. Parasympathetic stimulus when mastication is crucial to increase the velocity of saliva’s flow, which will stimulate the polypeptide to show. In human, defensins polypeptide has two subfamilies, the Alpha defensins (HNP1-3) and Beta defensins (hBD-2) which interact with different targets, meanwhile hBD-2 specially interacts with lipopolysaccharide (LPS). This research aim to explain the connection between mastication with increased content of hBD-2, HNP1-3and also the decrease amount of Streptococcus sanguis bacteria on gingivitis. The method of this research is experimental with posttest design observation to the patient of gingivitis. The subject of this research are 42 man from 17-22 years of age, devided to 3 groups. Each group consists of 14 men (healthy group-mastication; group of gingivitis- mastication; group of gingivitis-no mastication). Mastication used standard gums (equal size, without sweetener and flavor) and it is done for a week. Every time subjects wake up in the morning and the mastication is done for one minute (32 times). The subject’s saliva sample is contained in the Eppendorf Tube, which has been given Phenylmethylsulfonyl Fluoride (PMSF) 0,5 ml before. The test result of Elisa content hBD-2 shows the striking difference of the three groups, HNP1-3 content only shows the difference in group 1:2 and group 1:3. Real Time Polymerase Chain Reaction (RT PCR) shows quantity of Streptococcus sanguis which showed the difference between group 1:3 and group 2:3 About mastication’s role which caused the increase content of hBD-2 shows the meaningful relation in decreasing the quantity of bacteria is only to Streptococcus sanguis