Activity detection of noni leaf (Morinda citrifolia L.) chlorophyll extract through the photoinactivation to reducing Streptococcus mutans cells using XTT assay method


Sri Dewi Astuty, - and Syamsir Dewang, - and Fikhri Astina Tasmara, - and Rahmadan Riski Wahyudi, - Activity detection of noni leaf (Morinda citrifolia L.) chlorophyll extract through the photoinactivation to reducing Streptococcus mutans cells using XTT assay method. AIP Conference Proceedings 2663, 020001 (2022).

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Abstract (Abstrak)

Noni plant (Morinda citrifolia) has proven to contain antimicrobial substances, and inside their leaves have chlorophyll pigment. Today, Chlorophyll compound is a new candidate as a photosensitizer agent that is applied in the field of photodynamic inactivation (PDI) based on its natural properties, chemical purity, and quantum yield which has a long lifetime at the triplet excitation level. In this study, the PDT mechanism used a red laser (634 nm) and a blue laser (415 nm) in vitro which was applied to the planktonic Streptococcus mutans microbe culture with cell reduction analysis by comparing the XTT assay and CFU/mL test methods. The variations in the duration of the irradiation selected were 60, 120, 180, 240, and 300 seconds. The results showed that the aPDT-red laser-noni treatment was optimum with an exposure duration of 300 seconds (51%), while the aPDT-blue laser-noni treatment with an exposure duration of 300 seconds (62%). This indicates that the effectiveness of PACT against Streptococcus mutans with noni leaf extract as a photosensitizer is more effective when irradiated by a 415 nm laser than by a 634 nm laser

Item Type: Article
Subjects: Q Science > QC Physics
Depositing User: - Andi Anna
Date Deposited: 09 Dec 2022 02:12
Last Modified: 09 Dec 2022 02:12
URI: http://repository.unhas.ac.id:443/id/eprint/23858

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