EFEK RESTRIKSI KALORI DAN PUASA INTERMITTENT TERHADAP PROFIL LIPID DAN BERAT BADAN TIKUS WISTAR JANTAN = EFFECTS OF CALORIE RESTRICTION AND INTERMITTENT FASTING ON LIPID PROFILE AND WEIGHT MALE WISTAR RATS

NURLIANA. Efek Restriksi Kalori dan Puasa Intermittent terhadap Profil Lipid dan Berat Badan Tikus Wistar Jantan (dibimbing oleh Syahrijuita Kadir dan Ika Yustisia)
Penuaan adalah penurunan fungsional fisiologis bergantung pada waktu yang mepengaruhi organisme hidup. Obesitas merupakan faktor resiko utama penyakit serius yang diderita lansia seperti kardiovaskuler dan diabetes. Obesitas berkaitan dengan ketidakstabilan profil lipid dan berat badan. Restriksi kalori merupakan pengurangan asupan makanan tanpa malnutrisi. Upaya preventif dapat dilakukan dengan restriksi kalori, yang memiliki berbagai macam rejimen seperti restriksi kalori mimetik dan puasa intermittent. Penelitian ini bertujuan untuk mengetahui efek restriksi kalori, restriksi kalori mimetik, dan puasa intermittent terhadap profil lipid dan berat badan pada tikus Wistar tua jantan.
Teknik sampling menggunakan simple random sampling, dengan metode pre and post test with controlled group design. Sampel penelitian berjumlah 24 ekor tikus Wistar jantan yang dibagi menjadi 4 kelompok yaitu KO (kontrol), RK (restriksi kalori), RM (restriksi kalori mimetik), dan PI (puasa intermittent). Hasil penelitian setelah 30 hari perlakuan, tikus masih sehat dan beraktifitas normal serta ada penurunan berat badan pada kelompok RK(p<0.05) dan PI (p<0.05). Pada kadar trigliserida terjadi penurunan pada kelompok KO dan RM (p<0.05). Kadar kolesterol total terjadi penurunan pada kelompok KO, RK, RM dan PI (p<0.05). Kadar kolesterol LDL mengalami penurunan pada kelompok KO dan PI (p<0.05). Kadar kolesterol HDL mengalami peningkatan pada kelompok KO, RK, dan PI (p<0.05). Restriksi kalori memberikan efek yang baik terhadap kestabilan berat badan dan profil lipid, namun dari penelitian ini diketahui puasa intermittent memberikan pengaruh signifikan pada kolesterol total, kolesterol LDL, dan kolesterol HDL dibandingkan kelompok lainnya.

NURLIANA. Effects of Calorie Restriction and Intermittent Fasting on Lipid Profile and Weight of Male Wistar Rats (supervised by Syahrijuita Kadir dan Ika Yustisia)
Aging is decrease physiological function depending on time that affects living organisms. Obesity is a major risk factor for serious diseases suffered by the elderly, such as cardiovascular and diabetes. Obesity is associated with an unstable lipid profile and body weight. Calorie restriction is a reduction in food intake without malnutrition. Preventive efforts can be carried out by calorie restriction, which has a variety of regimens such as mimetic calorie restriction and intermittent fasting. This study aims to determine the effect of calorie restriction, mimetic calorie restriction, and intermittent fasting on lipid profiles and body weight in old male Wistar rats.
The sampling technique used simple random sampling, with method of pre and post test with controlled group design. The research sample consisted of 24 male Wistar rats which were divided into 4 groups, namely KO (control), RK (calorie restriction), RM (mimetic calorie restriction), and PI (intermittent fasting).
The results of the study after 30 days of treatment, the rats were still healthy and had normal activities and there was a decrease in body weight in the RK (p<0.05) and PI (p<0.05) groups. There was a decrease in triglyceride levels in the KO and RM groups (p<0.05). Total cholesterol levels decreased in the KO, RK, RM and PI groups (p<0.05). LDL-cholesterol levels decreased in the KO and PI groups (p<0.05). HDL-cholesterol levels increased in the KO, RK, and PI groups (p<0.05). Calorie restriction has a good effect on weight stability and lipid profile, but from this study it is known that intermittent fasting has a more significant effect on total cholesterol, LDL-cholesterol, and HDL cholesterol than the other groups.
Kata Kunci : restriksi kalori, restriksi kalori mimetik, puasa intermittent, profil lipid, dan penuaan.

Keywords: calorie restriction, mimetic calorie restriction, intermittent fasting, lipid profile, and aging.