KEMAMPUAN TUMBUH ISOLAT BAKTERI ASAM LAKTAT DARI AYAM KAMPUNG Gallus domesticus DAN ITIK BETINA Anas domesticus DENGAN PENGGUNAAN BAHAN PENYALUT SKIM MILK = GROWTH ABILITY OF LACTIC ACID BACTERIA ISOLATES FROM CHICKEN Gallus domesticus AND FEMALE DUCK Anas domesticus WITH THE USE OF SKIM MILK COATING MATERIAL

ASTRI ALFRIANTI. Growth Ability of Lactic Acid Bacteria Isolates from Free- Range Chickens Gallus domesticus and Female Ducks Anas domesticus Using Skim Milk as a Coating Agent, (supervised by Prof. Dr. Dirayah Rauf Husain, DEA). Baground. Lactic acid bacteria (LAB) play a crucial role in the food and health sectors due to their ability to produce bioactive metabolites and are excellent probiotic candidates. However, maintaining their viability and stability, especially for long-term use, requires appropriate conservation methods. Objectives. Analyzing the growth ability and generation time of LAB isolates from native chickens (PATA-5) and female ducks (PRIB-3), after conservation using skim milk coating material. Methods. This study consisted of 5 stages, namely: 1) Characterization of LAB isolates through purity tests, morphological observation, physiological tests, and biochemical tests; 2) Biomass production based on growth curves; 3) Inhibitory test againts pathogenic bacteria; 4) Conservation of LAB isolates through coating using skim milk, lyophilization for 49 hours, and storage at room temperature for one week; 5) Viability test. Results. LAB isolates PRIB-3 and PATA-5 showed good growth ability after conservation, with optimal biomass increase at 6 to 10,5 hours. Inhibition test showed that the inhibition zone of PRIB-3 lyophilisate ranged from 9-13.5 mm, while PATA-5 ranged from 10-12 mm. Both isolates were classified as having strong to very strong inhibitory activity. The generation time of PRIB-3 was recorded at 4 hours 30 minutes, while PATA-5 was 4 hours 15 minutes, which is still within the normal range for LAB and not significantly different compared to before conservation. Conclusion. LAB isolates PRIB-3 and PATA-5 had good growth ability after conservation using skim milk as a coating material.