The effect of zonation in the closed house broiler on the litter Escherichia coli count and ammonia concentration

The closed house is designed in length model form to provide differences in temperature, humidity and wind speed between the zone close to the inlet and the zone far from the inlet. This difference in environmental conditions has not been widely studied, especially for the growth of microorganisms present in the litter, especially Escherichia coli from chicken faeces contamination. Escherichia coli is a Gram-negative bacteria that normally occurs in the digestive tract and will be pathogenic if the numbers are excessive and outside the digestive tract. The purpose of this study was to determine the total E. coli bacteria in litter zoning in closed house facility. This is very important to be used as a reference in considering the proper litter handling. In this study, the closed house enclosure is formed 4 zones (zone 1 is 0-30 m from the inlet, zone 1 is 30-60 m from the inlet, zone 3 is 60-90 m from the inlet, and zone 4 is 90-120 m from the inlet. inlet). Each zone is 20×30 meters in size. The research was conducted using a sample of litter by taking 10 gr from each zone. The samples were then planted on EMBA (Eosin Methylene Blue Agar) media based on SNI 19-2897: 1993 work guidelines. The results showed that the total average of E. coli bacteria in zone 1 was 5.3×107 CFU/g, 2.5×107 CFU/dg in zone 2, 3.8×107 CFU/g in zone 3, and 5.4×107 CFU/g in zone 4. These results indicated that zoning did not affect the total E. coli bacteria on the litter so that it can be concluded that environmental conditions in the form of temperature, humidity, and wind speed in the zoning in the closed house were quite even. During the maintenance period, litter scraping was performed on the 7th day in zones 1 and 2, the 14th day in zones 3 and 4, and the 21st day in zones 1 and 2. On the 21st day, the litter bed is reversed in zone 3 and 4. Then add new husks on the 28th day. The conclusion showed that the treatment was effective in reducing the total E. coli bacteria in litter