DETERMINATION OF SECONDARY METABOLITES, TOXICITY AND ANTIOXIDANT ACTIVITIES OF BARK EXTRACTS OF Artocarpus lanceifolius ROXB

The purpose of study to determine the content of secondary metabolites, toxicity and antioxidant activity of bark extract from Artocarpus lanceifolius Roxb. The extracts were obtained by maceration method using hexane, chloroform, ethyl acetate and methanol solvents, respectively. The extract obtained was tested for phytochemicals, toxicity test using the shrimp Brine Lethality Test (BSLT), antioxidant test using the DPPH (2,2-diphenyl-1- picrylhydrazyl) method. Phytochemical test results showed that n-hexane extract contained terpenoids and steroids while flavonoid and phenol compounds contained in extracts of chloroform, ethyl acetate, and methanol. The dominant alkaloid compounds were found in ethyl acetate and methanol extracts. Toxicity values (LC50) for n-hexane, chloroform, ethyl acetate extract were 1.0853 μg/mL, 0.1635 μg/mL, 0.3615 μg/mL and 0.2609 μg/mL respectively. The IC50 value of free radical scevange activity of chloroform extract, ethyl acetate were 19.66 μg/mL, 15.85 μg/mL, and 19.01 μg/mL respectively.