The effect probiotics from the types of nuts in feeding to the for bacterial populations, enzyme activity and feed FCR for milkfish (Chanos chanos)

Nuts contain oligosaccharides which can function as prebiotics. Prebiotics are fibers
that the body cannot digest and become food for probiotics. This study aimed to analyze the
bacterial population, enzyme activity and feed conversion ratio of milkfish after being given
various prebiotics from legumes in the feed. Milkfish measuring 4.13 ± 2.75 g are reared in a
glass aquarium measuring 50 x 45 x 45 cm filled with water with a salinity of 15-20 ppt. Fish
are maintained at a density of 15 individuals /aquarium for 60 days and are fed 5% body
weight/day. The study was designed in a completely randomized design with 5 prebiotic
treatments of soybeans, peanuts, green beans, kidney beans, and control. The results showed
that the treatment had a significant effect (p <0.05) on the bacterial population, as well as the
protease and amylase enzyme activity, but not on FCR. The highest bacterial population was
red bean prebiotics (2,082 × 103 CFU / mL), followed by green beans (2,001 × 103 CFU/mL),
soybeans (972 × 103 CFU/mL). The highest activity (0.243 u/mL) and amylase enzyme (0.745
u/mL) was the prebiotic kidney beans, followed by soybeans (0.236 u/mL) amylase enzyme
(0.506 u/mL), peanuts, protease enzymes (0.129 u / mL) amylase enzyme (0.554 u/mL) and
green bean protease enzyme (0.156 u/mL) amylase enzyme (0.543 u/mL). Meanwhile, the FCR
ranges from 1.43 ± .0.85 to 2.87 ± .1.70. It can be concluded that the best type of prebiotic is
red beans.