DETECTING DNA POLYMORPHISM ON MULBERRY (Morus Sp.) USING RAPD AND ISSR MARKERS

BIMA AKZAD, MUHAMMAD and NURAENI, SITTI and HALIMAH LAREKENG, SITI (2021) DETECTING DNA POLYMORPHISM ON MULBERRY (Morus Sp.) USING RAPD AND ISSR MARKERS. Plant Cell Biotechnology and Molecular Biology.

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Abstract (Abstrak)

Mulberry molecular analysis in Indonesia has not been widely reported, so research towards molecular genetics needs to be done. Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) markers are reliable methods for identifying varieties by DNA polymorphism analysis to reveal genetic diversity of three provenances in South Sulawesi. This study aims to see the opportunities for polymorphisms using RAPD and ISSR markers in mulberry plants so that it is considered very important to be used as basic information in mulberry plant breeding and conservation programs in the future. The results of primary selected using RAPD markers on mulberry. Successfully amplified 8 out of 10 primers with clear polymorphic bands, namely primers OPK-20, OPA-15, OPA-05, OPAD-11, OPG-19, OPAE- 11, OPP-08, and OPD-20. Whereas, the primary ISSR 10 markers, namely UBC 810, UBC 813, UBC 814, UBC 820, UBC 822, UBC 823, UBC 824, UBC 827, UBC 830, and UBC 868 were successfully amplified with polymorphic and bright bands so that they can be used for diversity analysis for mulberry.

Item Type: Article
Subjects: S Agriculture > SD Forestry
Depositing User: - Andi Anna
Date Deposited: 12 Jul 2021 05:29
Last Modified: 12 Jul 2021 05:29
URI: http://repository.unhas.ac.id/id/eprint/5359

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