The quality of intact plasma membrane of bull frozen sperm in different breeds

Hypoosmotic Swelling (HOS) Test is a technique to evaluate the intact plasma membrane of spermatozoa. The purpose of this study was to determine the level of intact plasma membrane in various breeds of cattle regardless of age, feed, environment and maintenance management. Semen examination was carried out microscopically. Valuable semen assessment parameter was the percentage of intact plasma membrane in the cows of Bali, Limosin, FH, Brahman, Angus and Simental. Cattles with the highest intact plasma membrane values were Simental and the lowest was FH. The results of statistical analysis on the intact plasma membrane spermatozoa showed a significant difference (P<0.05) between cattle breeds. The intact plasma membrane in Simental was significantly different (P<0.05) compared to Bali, Limousine and FH. However, it is not significantly different from the Brahman and Brangus cattle. Different breeds of cattle can affect the level of intact plasma membrane.