Supplementation of Moringa (Moringa oleifera) seed extract in an extender on the quality of Bali bull semen

In the processing semen, there is a contact between semen and oxygen (O2 ) to produce free radicals which can reduce the quality of semen. The decrease in semen quality caused by free radicals can be suppressed by the addition of antioxidants in the extender. This study aimed to prove that the Moringa seed extract can be used as an alternative to natural ingredients that can be added to the semen extender, in which it can maintain the quality of Bali bull sperms at equilibration. This study was used one Bali bull for semen collection with the treatment of Moringa (Moringa oleifera) seed extract supplementation into Tris egg yolk (TEY) as an extender; 25% (P1), 50% (P2), 75% (P3). Andromed (P0A) and TEY 100% (P0B) were used as control with three replications. The parameters measured included the individual and progressive motility of the sperms. Each parameter in each treatment was compared using a factorial completely randomized design. The results showed that the use of Moringa seed extract supplemented in the extender resulting both individual and progressive motility of Bali bull sperms decreased slowly in comparison to those without supplementation. The optimum supplementation of Moringa seed extract at 25% could minimize the rate of decrease in sperms motility of Bali bull semen after equilibration.