GENETIC DIVERSITY OF EIGHT TYPES OF BAMBOO BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS

Bamboo is a plant that has been widely known to Indonesian people, generally used as a substitute for wood to supply the needs of the building and furniture raw materials. This study used molecular markers because these markers are stable and not affected by the environment. The purpose of this study was to determine the genetic diversity of eight types of bamboo in the Area of Genetic Resources (ASDG) of 2nd regional of Seed/Seedling Forest Center (BPTH) Bellabori Village, Parangloe District, Gowa Regency, Indonesia. Its usefulness is as information and reference for the development of ASDG Bamboo and also to support the conservation of Bamboo species. The analysis was carried out using 99 DNA samples from 8 Bamboo types. Primer selection using 12 randomly selected DNA samples produced four polymorphic primers out of 20 RAPD primers. RAPD primers produced polymorphic bands were OPP-08, OPA-15, OPC-11, and OPA-05 primers. Based on the analysis of genetic diversity, the average diversity was 0.47. Individuals observed from 99 samples tended to cluster according to their types. Thus genetic diversity for each individual was high.