HUBUNGAN MINOSIKLIN TERHADAP KADAR MATRIX METALLOPROTEINASE 9 (MMP-9) PADA KASUS CEDRA KEPALA TRAUMATIKA PADA TIKUS RATTUS WISTAR = THE EFFECT OF MINOCYCLINE ON MATRIX METALLOPROTEINASE 9 (MMP-9) LEVEL IN TRAUMATIC BRAIN INJURY IN WISTAR RATS

INTRODUCTION: Cerebral edema in traumatic brain injury (TBI) is caused by hyperpermeability of the blood-brain barrier (BBB). One of the causes of impaired BBB permeability is the activation of matrix metalloproteinase 9 (MMP-9), which can be inhibited by the antibiotic minocycline. This study aimed to investigate MMP-9 levels and the effect of minocycline administration in a Wistar rat model of traumatic brain injury.
METHODS: A modified Marmarou model of brain injury in rats was used in this study. The post-test control group design consisted of an untreated control group (group 1), a group with traumatic brain injury (group 2), and an injury group that received minocycline (group 3). Sandwich ELISA was used to evaluate MMP-9 levels.
RESULTS: The mean level of MMP-9 in group 2 was 0.610 ng/ml, compared with 0.519 ng/ml in the controls, and 0.552 ng/ml in group 3. Statistical analysis showed a statistically significant relationship between the administration of minocycline and the decrease in MMP-9 levels in Wistar rats with traumatic brain injury (p = 0.001).
CONCLUSION: Administration of minocycline to Wistar rats with traumatic brain injury resulted in significantly lower levels of MMP-9. This result showed that minocycline has the potential to inhibit increased MMP-9 levels in traumatic brain injury.

KEYWORDS: Traumatic brain injury; Minocycline; MMP-9; Experimental study.