DAYA HAMBAT FRAKSI ETANOL SARANG SEMUT (MYRMECODIA PENDANS) PADA SEL KANKER BURKITT’S LYMPHOMA (INVITRO) MELALUI HAMBATAN ANGIOGENESIS INTERLEUKIN 8 (IL-8)

Burkitt’s Lymphoma is a type of Non-Hodgkin Lymphoma cancer
that originates from B. Burkitt’s Lymphoma lymphocytes with a high gradation
ability formed from small, noncleaved, undifferentiated, and diffuse cells.
Burkitt’s Lymphoma is a proliferation and multiplication of cells that is very
aggressive thus people usually die quickly. Purpose: This study aims to know
how the inhibitory power of angiogenesis interleukin 8 ethanol fraction of ant nest
extract in Burkitt's lymphoma cancer cell culture. Methode: The study was
conducted in a pure laboratory experimental method using Burkitt's lymphoma
cancer cell cultures. Gradual research began with determination, extraction,
fractionation, making DMEM medium, activation of Burkitt’s Lymphoma cancer
cells, up to cytotoxicity test and angiogenesis inhibition test of flavonoid fraction
extract of ant nests. Data were analyzed using two-way ANOVA followed by Post
Hoc LSD (Least Significant Difference) test with a significance level of 95%.
Results : Cytotoxicity test results showed that the flavonoid ethyl acetate fraction
at a concentration of 1000 μg / mL could cause cell death as much as 82.71%, and
at the lowest concentration of 7.8125 μg / mL can cause cell death by 39.75%
cells. The results of the angiogenesis inhibition test showed a significant change
in the expression of interleukin 8 in standard solutions. Changes in the expression
of interleukin 8 in ethanol solution and ethyl acetate solution with a concentration
of 0 to a concentration of 125 results obtained continued to increase. However,
when the ethanol solution with 250 levels, the results obtained decreased from
0.254 to 0.237. Likewise, changes in the expression of interleukin 8 in ethyl
acetate solution with a concentration of 250 results obtained decreased from 0.291
to 0.261.