Analysis of the changes power output and energy dose to green laser against OD and MDA values after photoinactivation at Candida Albicans and Staphyloccocus epidermidis associate biofilms

Photodynamic Therapy (PDT) or Photodynamic Inactivation (PDI) to kill the pathogenic microbe like Candida albicans and Staphylococcus epidermidis has been more researches. The photoinactivation mechanism utilizes the activation process of sensitizer molecules by light so that an excitation process occurs and gets some reactive compounds such as singlet oxygen. The absorption of a number of the photon energy of light which is called the radiation energy dose which depends on the power output of the light source over a certain period of time. This study aims to compare the effect of photoinactivation on the biofilm associated C. albicans and S. epidermidis after exposure 60 to 300 seconds with two different output power a laser. The light instrument had been designed to use a microcontroller with a green laser completed of the display unit of output power. The results showed that PDI treatment with green laser was able to reduce biofilm cells up to 66.23% (P1 treatment) and 71.37% (P2 treatment). The results of the malondialdehyde (MDA) test showed that the highest value in the PD5 group was (0.389±0.004) nmol/mL. This result is lower than other research because the photoinactivation against microbes associate biofilm is more difficult than a single microbe.