The Analysis of Potency of Castor Leaf Extract (Jatropha Curcas L.) After Radiating with A Red Laser to Inhibit the Growth of Oxygenated Staphylococcus Epidermidis Biofilm

Photoinactivation known as Antimicrobial Photodynamic Therapy (aPDT) is part of the Photodynamic Therapy (PDT) system which is intended to inhibit the growth of pathogenic microbes that trigger infectious diseases in the human body. Photoinactivation developed in research has used natural photosensitizer agents extracted from plants that contain antimicrobial substances. This study aims to determine the optimal performance of castor leaf extract through irradiation with a red laser to kill Staphylococcus epidermidis biofilm cells whose oxygen level has been increased previously. The analytical method used is XTT staining assay with the Optical Density (OD) value as an indicator of the number of cells still active metabolize. The results obtained showed that the OD value for control group (P-) about (1.644±0.138), established a decrease after 10 minutes of irradiation of (0.926±0.220). This shows that the effectiveness of castor leaf extract after being activated by a high-potential red laser inhibits the growth of S. epidermidis biofilm cells.