Comparison of Fresh and Cryopreserved Semen Quality of Polled and Horned Bali Bulls

A purpose of study is to examine sperm quality of Bali polled and horned bulls. The semen samples col- lected from 4 Bali polled bulls and 4 horned bulls aged between 5 and 7 years using an artificial vagina, then evaluated macroscopically and microscopically. A macroscopic evaluation revealed no significant differences of semen between polled and horned cattle. However, the microscopic evaluation revealed that sperm concentration, motility, and abnormality of polled bulls were significantly lower than the horned bulls, and there was no apparent difference between viability and membrane integrity. Except for abnor- malities and DNA integrity of spermatozoa, all parameters of sperm quality were not significantly different between polled and horned bulls. Sperm abnormalities in polled bulls were significantly higher than in horned ones. However, sperm DNA integrity of the polled bulls was significantly higher than horned bulls. The analysis of CASA revealed that most kinematics were higher in polled bull spermatozoa than in horned ones, except for wobble (WOB) or ration of velocity average path (VAP) and velocity curvilinear (VCL) and amplitude of lateral head displacement (ALH), which were higher in horned bulls than in polled bulls. We therefore concluded that the quality of fresh sperm from horned bulls was better than polled bulls. However, polled bulls semen has greater freezing ability than the horned ones. Furthermore, the polled and horned bulls had the same semen characteristics that enable them for fertilizing ability, and both bulls have good DNA integrity.