The Effectiveness of Moringa Leaf Extract (Moringa Oleifera)Against Porphyromonas gingivalis Bacteria in Periodontitis Cases Through IL-1 Cytokine Analysis

Periodontitis is caused by bacteria that adhere to and grow on the tooth surface. The “red complex” bacteria consisted of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia. These bacteria will trigger an inflammatory response in the body. Interleukin 1 is an interleukin that acts both as a pro-inflammatory cytokine, Interleukin 1 is a stimulator for MMP production. Treating periodontal disease can be done through non-surgical therapy as well as surgical therapy, to maximize therapy it is accompanied by antimicrobial therapy, but because of the frequent use of antimicrobials causing antimicrobial resistance in patients so that the use of natural ingredients as additional therapy is very necessary in this study using leaves. Moringa as a substitute for antimicrobials.
This study aimed to determine the effectiveness of Moringa leaf (Moringa oleifera) in influencing the anti- inflammatory cytokine IL-1. The first benefit of this research is to provide scientific information in the field of dentistry regarding the effectiveness of Moringa leaves against red complex bacteria Porphyromonas gingivalis as a cause of chronic periodontitis through anti- inflammatory cytokine analysis.
The type of research that will be used is quasi-experimental with a post-test research design with a control group design. This study used a sample consisted of 30 Wistars (Rattus Novergicus) and was divided into 2 groups based on periodontal tissue sampling as follows treatment group which were treated with extracts Moringa and control group with aquadest irrigation after bacterial induction in the gingival sulcus. Blood samples were taken on days 0,1,3,5,7 and centrifuged obtain blood serum and serum cytokine levels (pg/mL) were quantified using a commercial ELISA IL-1 kit.
There is a significant difference in the levels of IL 1 on which day with the p value 0.000 (p<0,05). Moringa leaf extract can reduce pro-inflammatory cytokine IL 1 cells, seen after being given treatment using Moringa extract on the day of observation from D0, D1, D3, D5 and D7 in experimental animals wistar rats and induced using Porphyromonas gingivalis bacteria. Experimental article (J Int Dent Med Res