Motility, Viability, and Abnormality of the Frozen Bali Bull Semen with Andromed and Egg Yolk-Tris Extender

This study aimed to compare the quality of frozen semen (motility, viability and abnormality of the sperms) of Bali bull using Andromed and tris egg yolk (TEY) extenders. The semen of one Bali bull was collected using an artificial vagina. The semen was collected five times, in which, each semen collection was divided into two different extender treatments. Treatment 1 (T1) was Andromed+aquadest and T2 was TEY+glycerol. The parameters measured in the study were motility, viability, and abnormalities of the Bali bull post-thawing sperms. Each parameter in each treatment was compared using the T-test (t-test independent sample). This studyindicated that the quality of post-thawing semen in T1 was much better incomparison toT2;the motility and viability ofpost-thawing semen using Andromed were still above 40% (50.80±6.14% and 54.20±5.03%). On the other hand, the motility and viability of post-thawing semenin TEYwas25.80±4.43% and 33.30±2.64%, respectively. In conclusion, the used of
Andromed as an extender could maintain the motility and viability of the Bali bull sperms after thawing in comparison to TEY. However there was no different in sperms abnormality within the two extenders (13.20±2.73% vs 14.30±3.51).