Stimulation of Osteoblast and Osteocalcin in the Bone Regeneration By Giving Bonegraft Golden Sea Cucumber

Regeneration therapy in periodontal defects and requires a material that is biocompatible, especially in the form of bone graft material, to reduce pocket depth, restore attachment, fill bone defects, and regenerate new bones, cementum, and periodontal ligaments. Golden sea cucumber (Stichopus hermanni) contains various bioactive components that are beneficial for human health. Objectives is to analyze the process of bone regeneration after the administration of bone graft Golden Sea cucumber through an increase in the number of Osteoblasts and Osteocalcin. This study used raw material in Golden Sea cucumber. After going through the process of deproteinization and maceration, performed XRD and XRF test analysis. The study was an experimental laboratory with the subject being a male guinea pig. The number of samples in this study was thirty guinea pigs. After 14 and 21 days, guinea pig sacrificed for the histological examination and immuno-histochemistry. Expression of Osteoblasts and Osteocalcin in the Golden Sea cucumber group significantly differ from the positive control group. Bone graft material from golden sea cucumber has the bone regeneration mechanism as bone graft material, so golden sea cucumber can be used as an alternative material for bone regeneration.