Histopathology overview of the liver of sulawesi medaka fish (oryzias celebensis) infected by vibrio cholera bacteria

This study aims to determine the histopathological analysis of the liver of Sulawesi medaka fish (Oryzias celebensis) infected with Vibrio cholerae. The sample was divided into three groups, namely group 1 as a negative control group (G1) without treatment, group 2 namely the LD50 treatment group (G2) by giving V. cholerae bacteria 0.02 ml with a concentration of 5.73 × 105 CFU/ml into 600 ml water, group 3 as the experimental dosage treatment group (G3) with the administration of 0.2 ml V. cholerae bacteria with a concentration of 6 × 107 CFU/ml into 600 ml water. After soaking the bacteria for 5 hours, the fishes were kept in containers and maintained for seven days, and then on the 7th day samples were taken and histological examination were carried out. No symptoms were observed in any group of fishes with in 24 hours. From day 3rd fishes starts showing clinical signs like solitary swimming, weakness and swimming with the body upside down on the surface of the water and on day seven more than half of the fish population with bacterial immersion treatment is found dead. Histological examination results revealed no pathological changes in group G1 infected with V.cholerae, in group G2 there found necrosis, fat degeneration, hemorrhage and melanomacrophage. In contrast, in group G3 histological examination revealed more severe degenerations, hemorrhages and necrosis as compared to other two groups.