ANALISA EKSPRESI GEN mRNA YY1 dan GEN mRNA p53 terhadap TNM STADIUM KARSINOMA NASOFARING

Nasopharyngeal cancer (NPC) is a malignant disease in the head and neck and is the fifth cancer in the human body. Radiochemotherapy is the first choice for handling NPC. The protein gene 53 (p53) is a tumor suppressor gene. YinYang1 (YY1) is a ubiquitous & multifunctional zinc-finger transcription factor, which has an important role in cell cycle control. YY1 can function as an activator, repressor or initiator of the gene transcriptional process.
The purpose of this study was to determine the relationship between the expression of the 53 mRNA gene protein (p53) and the expression of the YY1 mRNA gene against NPC TNM Stage.
Material and methods cross-sectional research on 20 WHO3 NPC samples in the form of 3 samples after radiochemotherapy, 17 samples of non-radiochemotherapy is consist of 8 stage two TNM samples, 7 stage three TNM samples and 2 stage four TNM samples. With RT-PCR, YY1 mRNA gene expression and p53 mRNA gene expression were measured, then the independent T-test of the mean sample group radiochemotherapy.
YY1 mRNA gene expression in NPC post-radiochemotherapy has a mean value of 7.9096 compared to that which has never been radiochemotherapy with an average of 11,656. The results of this statistical T test have a p-value of 0.00069 <0.05. NPC Stadium that descended after radiochemotherapy found expression of the YY1 mRNA gene also dropped.
Expression of the p53 mRNA gene in NPC after radiochemotherapy had an average value of 12,924 compared with never before radiochemotherapy 8.341 The results of this statistical T test had a p-value of 0.0001 <0.05. TNM Stadium NPC that descended after radiochemotherapy was found to be higher expression of the p53 mRNA gene.
At higher NPC TNM stages, the level of YY1 mRNA gene expression was relatively higher. NPC Post-radiochemotherapy there was a high level of expression of the p53 mRNA gene compared to the non-radiochemotherapy group.
It was concluded that the increased expression of the p53 mRNA gene was associated with a decrease in the expression of the YY1 mRNA gene in post-radiopharyngeal cancer patients in which TNM Stadium was decreased.