EFFECT OF GREEN TEA EXTRACT ON MODULATING THE ANTIBACTERIAL ACTIVITY OF STANDARD ANTIBIOTICS AGAINST THE CLINICAL ISOLATES OF Acinetobacter baumannii

Most of the clinical isolates of Acinetobacter baumannii are found resistant to the β-lactam antibiotics. This research aimed to determine the ability of green tea extract in modulating the antibacterial activity of standard antibiotics amoxicillin, cefotaxime, and imipenem against the clinical isolates of A. baumannii. The clinical isolates used in this study were collected from the Laboratory of Clinical Pathology, Wahidin Sudiro Husodo Hospital Makassar, Indonesia. To determine whether the bacterial isolate is resistant, the experiment was carried out using disk agar diffusion and Vitek-2 methods. Further, the antibacterial activity of the green tea, selected antibiotics, and their combination was determined by using a checkerboard microdilution assay. Results of the study revealed that among the selected two clinical isolates one of the A. baumannii isolates was found resistant to selected standard amoxicillin-clavulanate, cefotaxime, and imipenem, while the other one was found sensitive. Further, green tea extract with a concentration of up to 1.2 mg/ml didn't have any significant effect on the inhibition of A. baumannii growth. Similarly, at the same concentration (1.2 mg/ml) no modulation effect of green tea extract was reported on the antibacterial activity of amoxicillin, cefotaxime, and imipenem against the A. baumannii isolates