Hasanuddin University

Occurrence and Molecular Identification of Anisakis Dujardin, 1845 from Marine Fish in Southern Makassar Strait, Indonesia

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dc.contributor.author Anshary, Hilal
dc.date.accessioned 2014-03-14T11:53:06Z
dc.date.available 2014-03-14T11:53:06Z
dc.date.issued 2014-02
dc.identifier.citation Science Citation Index en_US
dc.identifier.issn 1738-0006 (online)
dc.identifier.issn 0023-4001 (print)
dc.identifier.uri http://repository.unhas.ac.id/handle/123456789/9061
dc.description.abstract Anisakis spp. (Nematoda: Anisakidae) parasitize a wide range of marine animals, mammals serving as the definitive host and different fish species as intermediate or paratenic hosts. In this study, 18 fish species were investigated for Anisakis infection. Katsuwonus pelamis, Euthynnus affinis, Caranx sp., and Auxis thazard were infected with high prevalence of Anisakis type I, while Cephalopholis cyanostigma and Rastrelliger kanagurta revealed low prevalence. The mean intensity of Anisakis larvae in K. pelamis and A. thazard was 49.7 and 5.6, respectively. A total of 73 Anisakis type I larvae collected from K. pelamis and A. thazard were all identified as Anisakis typica by PCR-RFLP analysis. Five specimens of Anisakis from K. pelamis and 15 specimens from A. thazard were sequenced using ITS1-5.8S-ITS2 region and 6 specimens from A. thazard and 4 specimens from K. pelamis were sequenced in mtDNA cox2 region. Alignments of the samples in the ITS region showed 2 patterns of nucleotides. The first pattern (genotype) of Anisakis from A. thazard had 100% similarity with adult A. typica from dolphins from USA, whereas the second genotype from A. thazard and K. pelamis had 4 base pairs different in ITS1 region with adult A. typica from USA. In the mtDNA cox2 regions, Anisakis type I specimens from A. thazard and K. pelamis showed similarity range from 94% to 99% with A. typica AB517571/DQ116427. The difference of 4 bp nucleotides in ITS1 regions and divergence into 2 subgroups in mtDNA cox2 indicating the existence of A. typica sibling species in the Makassar Strait. en_US
dc.description.sponsorship This study was partially supported by Directorate General of Higher Education, Indonesia, through Indonesian National Strategic Research Grant (HIBAH STRANAS), grant no. 510/SP2H/PP/DP2M/VII/2010 (24 July 2010), and Academic Recharging Program conducted at The University of Tokyo, Japan en_US
dc.language.iso en en_US
dc.publisher Korean Society for Parasitology and Tropical Medicine en_US
dc.subject Anisakis typica, molecular identification, internal transcribed spacer, PCR-RFLP, Makassar Strait, Indonesia en_US
dc.title Occurrence and Molecular Identification of Anisakis Dujardin, 1845 from Marine Fish in Southern Makassar Strait, Indonesia en_US
dc.type Article en_US
dc.UNHAS.email hilalanshary@unhas.ac.id en_US
dc.UNHAS.email sriwulancinga@yahoo.com en_US
dc.UNHAS.Fakultas Fakultas Ilmu Kelautan dan Perikanan en_US
dc.UNHAS.Prodi Program Studi Budidaya Perairan en_US
dc.UNHAS.idno 196710121992021001 en_US
dc.UNHAS.idno 196606301991032002 en_US

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