Hasanuddin University

INHIBITION OF HISTAMINE FORMATION ON THE FRIGATE TUNA (Auxis thazard thazard, L) USING LEAF EXTRACT OF Jatropha curcas

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dc.contributor.author Firdaus
dc.contributor.author Setha, Beni
dc.contributor.author Laga, Amran
dc.contributor.author Mahendradatta, Meta
dc.date.accessioned 2017-11-14T08:09:21Z
dc.date.available 2017-11-14T08:09:21Z
dc.date.issued 2014-01
dc.identifier.citation google scholar, Thomson Reuters en_US
dc.identifier.issn 2320-5407
dc.identifier.uri http://repository.unhas.ac.id/handle/123456789/26229
dc.description This arcticle discuss about the potency of leaf extract Jatropha curcas to inhibit the formation of histamine on the frigate tuna (Auxis thazard thazard, L) en_US
dc.description.abstract The study of the inhibition of histamine formation on the frigate tuna using jatropha leaf extract has been done. Jatropha leaf macerated in stages ranging from hexane, chloroform, ethyl acetate and methanol. Results of preliminary research note that the ethyl acetate extract and methanol with a concentration of 1% (w / v) is the best extract that provides antibacterial activity against several of histamine-forming bacteria. In this experiment, frigate tuna were divided into 3 groups, group 1 was soaked for 30 minutes in water, group 2 soaked for 30 minutes in a solution of ethyl acetate extract of leaf jatropha with a concentration of 1%, and group 3 was soaked for 30 minutes in a solution of methanol extract of leaf jatropha with a concentration of 1%. After that, frigate tuna stored at room temperature for 5 hours, then analyzed the number of bacterial colony with total plate count method (TPC), number of histamine-forming bacteria and histamine levels. The results showed that the value of TPC the frigate tuna its soaked in water are log 6.68 (cfu / g), soaked in a solution of ethyl acetate extract are log 4.94 (cfu / g) and soaked in a solution of methanol extract are log 5.61 (cfu / g). The number of histamine-forming bacteria in the frigate tuna its soaked in water are log 5.62 (cfu / g), soaked in a solution of ethyl acetate extract are log 4.40 (cfu / g) and soaked in a solution of methanol extract are log 5.54 (cfu / g). histamine levels of the frigate tuna its soaked in water are 17.73 mg / kg, soaked in a solution of ethyl acetate extract are 11.07 mg / kg and soaked in a solution of methanol extract are 14.02 mg / kg. Thus, the ethyl acetate extract of leaves of Jatropha is more effective to inhibiting the growth of histamine-forming bacteria and inhibit the formation of histamine in fresh frigate tuna that stored for 5 hours at room temperature. en_US
dc.language.iso en en_US
dc.publisher Open access journal en_US
dc.relation.ispartofseries Vol. 2;Issue 1
dc.subject Histamine, frigate tuna, Jatropha curcas, en_US
dc.title INHIBITION OF HISTAMINE FORMATION ON THE FRIGATE TUNA (Auxis thazard thazard, L) USING LEAF EXTRACT OF Jatropha curcas en_US
dc.type Article en_US
dc.UNHAS.email firdaus@unhas.ac.id en_US
dc.UNHAS.Fakultas FMIPA en_US
dc.UNHAS.Prodi Kimia en_US
dc.UNHAS.idno 196009091988101001 en_US


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