Hasanuddin University

Rhodopsin promoter-EGFP fusion transgene expression in photoreceptor neurons of retina and pineal complex in mice

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dc.contributor.author Andi Muhammad Ichsan, Andi Muhammad Ichsan
dc.date.accessioned 2017-04-20T01:36:31Z
dc.date.available 2017-04-20T01:36:31Z
dc.date.issued 2005-05-06
dc.identifier.citation - en_US
dc.identifier.other doi:10.1016/j.neulet.2004.12.072
dc.identifier.uri http://repository.unhas.ac.id/handle/123456789/23979
dc.description - en_US
dc.description.abstract Light detection in vertebrate eyes is mediated through retinal photoreceptor rod and cone cells that transduce light signals into electrical responses. The differentiation and synaptogenesis of photoreceptor cclls are especially important since these cells arc the main targets of degeneration in retinitis pigmentosa. We produced transgenic mice that express enhanced green fluorescent protein (EGFP) under the control of the mouse rhodopsin gene promoter. In Western blot analyses of transgenic retinal homogenates, expression of the endogenous rhodopsin gene was detected from post-natal day (P)8; however, EGFP expression in transgenic retinas was initially detected at PI 2, indicating delayed pvnrpccirvn nf the fr^insgpnp At P145 TT—ZTZZZZ^y u *«vuk vAprcSiiOii uf LGi " ut iuv uoii^cia^ iii iltt. <uuu transgenic uiicc, the strongest EGFP expression was observed in the outer nuclear layer, and to a lesser extent in the outer plexiform layer as well as in thp inner and outer segments. F.GFP expression was also observed in the pineal stalk. The rhodopsin promoter-EGFP transgenic tnice will be not only useful to assess rhodopsin gene promoter activity in vivo, but also for retinal transplant studies as a source of functional photoreceptor cells that are fluorescent green. © 2005 Elsevier Ireland Ltd. All rights reserved. en_US
dc.description.sponsorship - en_US
dc.publisher Elsevier Ireland Ltd. en_US
dc.relation.ispartofseries volume 379,;no. 2
dc.subject Transgenic mice; EGFP; Photoreceptor; Rhodopsin; Promoter; Retina en_US
dc.title Rhodopsin promoter-EGFP fusion transgene expression in photoreceptor neurons of retina and pineal complex in mice en_US
dc.type Article en_US
dc.UNHAS.email am_ichsan@yahoo.com en_US
dc.UNHAS.Fakultas Kedokteran en_US
dc.UNHAS.Prodi kedokteran en_US
dc.UNHAS.idno 197002122008011013 en_US

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