Hasanuddin University

Identification of Actinomycetes from Marine Sediments with 
Different Environmental Characteristics

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dc.contributor.author Tahir, Akbar
dc.contributor.author Gosalam, Sulaiman
dc.date.accessioned 2014-09-04T15:16:18Z
dc.date.available 2014-09-04T15:16:18Z
dc.date.issued 2010-08-11
dc.identifier.citation Konas Pesisir VII en_US
dc.identifier.uri http://repository.unhas.ac.id/handle/123456789/10966
dc.description Hibah Dikti 2009 en_US
dc.description.abstract ABSTRACT Research was conducted for inventing active substance from marine Actinomycetes having antiviral activities on tiger shrimp (Penaeus monodon) with particular interest on the White Spot Syndrome Virus (WSSV). The WSSV (namely: white spot disease) could cause a 100% mortalities in only 3 – 10 days post infection, hence, a massive loss for the shrimp farmers. Actinobacteria is a class of microorganism which commonly known as Actinomycetes and belong to the family Actinomycetaceae and Gram-positive bacteria group. Actinomycetes are well known for their ability to produce secondary metabolites, many of which are active against pathogenic microorganisms, having high prophylactic and therapeutic values which made them attractive as a research object(s) for the bioactive compounds which functioned as anti-viral agents. This research was designed as an experimental survey in which sediments from different environmental characteristics (ie. mangrove area, river mouth/estuaria and small island surrounding at Kabupaten Pangkajene dan Kepulauan, South Sulawesi) were collected. The sediment were then aseptically dried, followed by spreading a thin layer of dehydrated sediment on agar media (seed culture), for upto 4 weeks. When these results were reported, three genera of actinomycetes being identified, based on the morphology characteristics such as the color of colony, presence of aerial and/or substrate mycellia, microscopical morphology of the spore surface and the coloration of media (ie. Streptomyces sp., Actinopolyspora sp dan Nocardia sp.). On the initial sediment cultures, it was also observed that the sediment from mangrove area yielded in more abundant actinomycetes colonies compared to other locations. After the colonies identification, the actinomycetes colonies were then propagated in broth media (production media), followed by rotapavor methanol extraction and remix with commercially available shrimp larvae feed (Ø ≤ 1 mm) with Bindex-gel. Post-larvae (PL20) of the tiger shrimp will be fed with these granules pellet containing actinomycetes extract until satiation for 14 consecutive days. The challenge test will be conducted by means of co-habitation the larvae with artificially WSSV infected shrimp (with homogenated organ/tissues of WSSV positive shrimps) for 96 hours and percentage of survival rate was recorded. Shrimp with normal feed were used as controls. en_US
dc.description.sponsorship DP2M-DIKTI en_US
dc.publisher Konas Pesisir VII Ambon en_US
dc.subject Actinomycetes, Mangroves, River Mouth, Small island en_US
dc.title Identification of Actinomycetes from Marine Sediments with 
Different Environmental Characteristics en_US
dc.type Article en_US
dc.UNHAS.email akbar.uba1@gmail.com en_US
dc.UNHAS.Fakultas Fak Ilmu Kelautan dan Perikanan en_US
dc.UNHAS.Prodi Ilmu Kelautan en_US
dc.UNHAS.idno 196107181988101001 en_US

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